Optimization of storage conditions for Puumala virus based vaccine

We report the effects of storage conditions and human serum albumin addition to vaccine composition on immunogenic properties an experimental against hemorrhagic fever with renal syndrome. To obtain vaccine, Puumala virus, propagated in Vero cells culture, was concentrated, inactivated beta-propiolactone, purified by chromatography CaptoCore 700 multimodal sorbent (GE Healthcare). The target component 20.2 106 viral RNA copies/ml. humoral immune response determined measuring neutralizing antibodies blood immunized Syrian hamsters (Mesocricetus auratus). It revealed that virus beta-propiolactone induces a pronounced response, which indicates preservation relevant epitopes. Аccording our study, is more stable during vs. intact virus. confirmed full inactivation occurred after 24 hours at 37C. A significant decrease immunogenicity thermal noted, confirms need for careful selection thermolabile Hantaviruses. direct correlation observed between titer copy number/ml. Adding stabilized infectivity storage: remained same level 3 months 62C, whereas without albumin, decreased undetectable level. 0.1% its long-term storage. shown preparations (0.11%) show higher dose-dependent stability upon repeated freezing assessed detecting their immunogenicity. can be assumed provides better genetic material storage, as well protein epitopes responsible protective form induction, undergo less conformational changes presence albumin. These data support formulation.